Part:BBa_K3136014:Design

J23100-B0034-GFP-ter-typeIIS-RBS-A

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 57
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal SpeI site found at 57
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 57
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 57
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

As the sacr between BioBrick RFC_10 and typeIIS assembly are different and may effect the protein expression, we constructed the four RBS to measure the expression strength.

Source

Artifial synthesis.